Kinesin-14 and kinesin-5 antagonistically regulate microtubule nucleation by γ-TuRC in yeast and human cells
Identifieur interne : 000144 ( Main/Exploration ); précédent : 000143; suivant : 000145Kinesin-14 and kinesin-5 antagonistically regulate microtubule nucleation by γ-TuRC in yeast and human cells
Auteurs : Zachary T. Olmsted [États-Unis] ; Andrew G. Colliver [États-Unis] ; Timothy D. Riehlman [États-Unis] ; Janet L. Paluh [États-Unis]Source :
- Nature Communications ; 2014.
Abstract
Bipolar spindle assembly is a critical control point for initiation of mitosis through nucleation and organization of spindle microtubules and is regulated by kinesin-like proteins. In fission yeast, the kinesin-14 Pkl1 binds the γ-tubulin ring complex (γ-TuRC) microtubule-organizing centre at spindle poles and can alter its structure and function. Here we show that kinesin-14 blocks microtubule nucleation in yeast and reveal that this inhibition is countered by the kinesin-5 protein, Cut7. Furthermore, we demonstrate that Cut7 binding to γ-TuRC and the Cut7 BimC domain are both required for inhibition of Pkl1. We also demonstrate that a yeast kinesin-14 peptide blocks microtubule nucleation in two human breast cancer cell lines, suggesting that this mechanism is evolutionarily conserved. In conclusion, using genetic, biochemical and cell biology approaches we uncover antagonistic control of microtubule nucleation at γ-TuRC by two kinesin-like proteins, which may represent an attractive anti-mitotic target for cancer therapies.
Url:
DOI: 10.1038/ncomms6339
PubMed: 25348260
PubMed Central: 4220466
Affiliations:
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Colliver</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State University of New York Polytechnic Institute, College of Nanoscale Science, Nanobioscience Constellation</institution>, Albany, New York 12203,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Riehlman, Timothy D" sort="Riehlman, Timothy D" uniqKey="Riehlman T" first="Timothy D." last="Riehlman">Timothy D. Riehlman</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State University of New York Polytechnic Institute, College of Nanoscale Science, Nanobioscience Constellation</institution>, Albany, New York 12203,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Paluh, Janet L" sort="Paluh, Janet L" uniqKey="Paluh J" first="Janet L." last="Paluh">Janet L. Paluh</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State University of New York Polytechnic Institute, College of Nanoscale Science, Nanobioscience Constellation</institution>, Albany, New York 12203,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25348260</idno><idno type="pmc">4220466</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4220466</idno><idno type="RBID">PMC:4220466</idno><idno type="doi">10.1038/ncomms6339</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000063</idno><idno type="wicri:Area/Pmc/Curation">000063</idno><idno type="wicri:Area/Pmc/Checkpoint">000122</idno><idno type="wicri:Area/Ncbi/Merge">000759</idno><idno type="wicri:Area/Ncbi/Curation">000759</idno><idno type="wicri:Area/Ncbi/Checkpoint">000759</idno><idno type="wicri:Area/Main/Merge">000144</idno><idno type="wicri:Area/Main/Curation">000144</idno><idno type="wicri:Area/Main/Exploration">000144</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Kinesin-14 and kinesin-5 antagonistically regulate microtubule nucleation by γ-TuRC in yeast and human cells</title><author><name sortKey="Olmsted, Zachary T" sort="Olmsted, Zachary T" uniqKey="Olmsted Z" first="Zachary T." last="Olmsted">Zachary T. Olmsted</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State University of New York Polytechnic Institute, College of Nanoscale Science, Nanobioscience Constellation</institution>, Albany, New York 12203,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Colliver, Andrew G" sort="Colliver, Andrew G" uniqKey="Colliver A" first="Andrew G." last="Colliver">Andrew G. Colliver</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State University of New York Polytechnic Institute, College of Nanoscale Science, Nanobioscience Constellation</institution>, Albany, New York 12203,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Riehlman, Timothy D" sort="Riehlman, Timothy D" uniqKey="Riehlman T" first="Timothy D." last="Riehlman">Timothy D. Riehlman</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State University of New York Polytechnic Institute, College of Nanoscale Science, Nanobioscience Constellation</institution>, Albany, New York 12203,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Paluh, Janet L" sort="Paluh, Janet L" uniqKey="Paluh J" first="Janet L." last="Paluh">Janet L. Paluh</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State University of New York Polytechnic Institute, College of Nanoscale Science, Nanobioscience Constellation</institution>, Albany, New York 12203,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Nature Communications</title><idno type="e-ISSN">2041-1723</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Bipolar spindle assembly is a critical control point for initiation of mitosis through nucleation and organization of spindle microtubules and is regulated by kinesin-like proteins. In fission yeast, the kinesin-14 Pkl1 binds the γ-tubulin ring complex (γ-TuRC) microtubule-organizing centre at spindle poles and can alter its structure and function. Here we show that kinesin-14 blocks microtubule nucleation in yeast and reveal that this inhibition is countered by the kinesin-5 protein, Cut7. Furthermore, we demonstrate that Cut7 binding to γ-TuRC and the Cut7 BimC domain are both required for inhibition of Pkl1. We also demonstrate that a yeast kinesin-14 peptide blocks microtubule nucleation in two human breast cancer cell lines, suggesting that this mechanism is evolutionarily conserved. In conclusion, using genetic, biochemical and cell biology approaches we uncover antagonistic control of microtubule nucleation at γ-TuRC by two kinesin-like proteins, which may represent an attractive anti-mitotic target for cancer therapies.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Rieder, C" uniqKey="Rieder C">C. Rieder</name></author><author><name sortKey="Khodjakov, A" uniqKey="Khodjakov A">A. Khodjakov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Riehlman, T R" uniqKey="Riehlman T">T. R. Riehlman</name></author><author><name sortKey="Olmsted, Z T" uniqKey="Olmsted Z">Z. T. Olmsted</name></author><author><name sortKey="Paluh, J L" uniqKey="Paluh J">J. L. Paluh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Manchado, E" uniqKey="Manchado E">E. Manchado</name></author><author><name sortKey="Guillamot, M" uniqKey="Guillamot M">M. Guillamot</name></author><author><name sortKey="Malumbres, M" uniqKey="Malumbres M">M. Malumbres</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pickett Heaps, J D" uniqKey="Pickett Heaps J">J. D. Pickett-Heaps</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oakley, C E" uniqKey="Oakley C">C. E. Oakley</name></author><author><name sortKey="Oakley, B R" uniqKey="Oakley B">B. R. Oakley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stearns, T" uniqKey="Stearns T">T. Stearns</name></author><author><name sortKey="Evans, L" uniqKey="Evans L">L. Evans</name></author><author><name sortKey="Kirschner, M" uniqKey="Kirschner M">M. Kirschner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moritz, M" uniqKey="Moritz M">M. Moritz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wiese, C" uniqKey="Wiese C">C. Wiese</name></author><author><name sortKey="Zheng, Y" uniqKey="Zheng Y">Y. Zheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luders, J" uniqKey="Luders J">J. Lüders</name></author><author><name sortKey="Stearns, T" uniqKey="Stearns T">T. Stearns</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nogales, E" uniqKey="Nogales E">E. Nogales</name></author><author><name sortKey="Wolf, S G" uniqKey="Wolf S">S. G. Wolf</name></author><author><name sortKey="Downing, K H" uniqKey="Downing K">K. H. Downing</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aldaz, H" uniqKey="Aldaz H">H. Aldaz</name></author><author><name sortKey="Rice, L M" uniqKey="Rice L">L. M. Rice</name></author><author><name sortKey="Stearns, T" uniqKey="Stearns T">T. Stearns</name></author><author><name sortKey="Agard, D A" uniqKey="Agard D">D. A. Agard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guillet, V" uniqKey="Guillet V">V. Guillet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kollman, J M" uniqKey="Kollman J">J. M. Kollman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Horio, T" uniqKey="Horio T">T. Horio</name></author><author><name sortKey="Oakley, B R" uniqKey="Oakley B">B. R. Oakley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Riehlman, T R" uniqKey="Riehlman T">T. R. Riehlman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Masuda, H" uniqKey="Masuda H">H. Masuda</name></author><author><name sortKey="Mori, R" uniqKey="Mori R">R. Mori</name></author><author><name sortKey="Yukawa, M" uniqKey="Yukawa M">M. Yukawa</name></author><author><name sortKey="Toda, T" uniqKey="Toda T">T. Toda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Olmsted, Z T" uniqKey="Olmsted Z">Z. T. Olmsted</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pidoux, A L" uniqKey="Pidoux A">A. L. Pidoux</name></author><author><name sortKey="Ledizet, M" uniqKey="Ledizet M">M. LeDizet</name></author><author><name sortKey="Cande, W Z" uniqKey="Cande W">W. Z. Cande</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saunders, W" uniqKey="Saunders W">W. Saunders</name></author><author><name sortKey="Hornack, D" uniqKey="Hornack D">D. Hornack</name></author><author><name sortKey="Lengyel, V" uniqKey="Lengyel V">V. Lengyel</name></author><author><name sortKey="Deng, C" uniqKey="Deng C">C. Deng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grishchuk, E L" uniqKey="Grishchuk E">E. L. Grishchuk</name></author><author><name sortKey="Spiridonov, I S" uniqKey="Spiridonov I">I. S. Spiridonov</name></author><author><name sortKey="Mcintosh, J R" uniqKey="Mcintosh J">J. R. McIntosh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cai, S" uniqKey="Cai S">S. Cai</name></author><author><name sortKey="Weaver, L N" uniqKey="Weaver L">L. N. Weaver</name></author><author><name sortKey="Ems Mcclung, S C" uniqKey="Ems Mcclung S">S. C. Ems-McClung</name></author><author><name sortKey="Walczak, C E" uniqKey="Walczak C">C. E. Walczak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rodriguez, A S" uniqKey="Rodriguez A">A. S. Rodriguez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Simeonov, D R" uniqKey="Simeonov D">D. R. Simeonov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paluh, J L" uniqKey="Paluh J">J. L. Paluh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tange, Y" uniqKey="Tange Y">Y. Tange</name></author><author><name sortKey="Fujita, A" uniqKey="Fujita A">A. Fujita</name></author><author><name sortKey="Toda, T" uniqKey="Toda T">T. Toda</name></author><author><name sortKey="Niwa, O" uniqKey="Niwa O">O. Niwa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mayer, C" uniqKey="Mayer C">C. Mayer</name></author><author><name sortKey="Filopei, J" uniqKey="Filopei J">J. Filopei</name></author><author><name sortKey="Batac, J" uniqKey="Batac J">J. Batac</name></author><author><name sortKey="Alford, L" uniqKey="Alford L">L. Alford</name></author><author><name sortKey="Paluh, J L" uniqKey="Paluh J">J. L. Paluh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hagan, I" uniqKey="Hagan I">I. Hagan</name></author><author><name sortKey="Yanagida, M" uniqKey="Yanagida M">M. Yanagida</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fu, C" uniqKey="Fu C">C. Fu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drummond, D R" uniqKey="Drummond D">D. R. Drummond</name></author><author><name sortKey="Hagan, I M" uniqKey="Hagan I">I. M. Hagan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Heck, M M S" uniqKey="Heck M">M. M. S. Heck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lawrence, C J" uniqKey="Lawrence C">C. J. Lawrence</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Enos, A P" uniqKey="Enos A">A. P. Enos</name></author><author><name sortKey="Morris, N R" uniqKey="Morris N">N. R. Morris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Syrovatkina, V" uniqKey="Syrovatkina V">V. Syrovatkina</name></author><author><name sortKey="Fu, C" uniqKey="Fu C">C. Fu</name></author><author><name sortKey="Tran, P T" uniqKey="Tran P">P. T. Tran</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schmidt, S" uniqKey="Schmidt S">S. Schmidt</name></author><author><name sortKey="Sohrmann, M" uniqKey="Sohrmann M">M. Sohrmann</name></author><author><name sortKey="Hofmann, K" uniqKey="Hofmann K">K. Hofmann</name></author><author><name sortKey="Woollard, A" uniqKey="Woollard A">A. Woollard</name></author><author><name sortKey="Simanis, V" uniqKey="Simanis V">V. Simanis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grallert, A" uniqKey="Grallert A">A. Grallert</name></author><author><name sortKey="Krapp, A" uniqKey="Krapp A">A. Krapp</name></author><author><name sortKey="Bagley, S" uniqKey="Bagley S">S. Bagley</name></author><author><name sortKey="Simanis, V" uniqKey="Simanis V">V. Simanis</name></author><author><name sortKey="Hagan, I M" uniqKey="Hagan I">I. M. Hagan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gachet, Y" uniqKey="Gachet Y">Y. Gachet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hagan, I" uniqKey="Hagan I">I. Hagan</name></author><author><name sortKey="Yanagida, M" uniqKey="Yanagida M">M. Yanagida</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tanenbaum, M E" uniqKey="Tanenbaum M">M. E. Tanenbaum</name></author><author><name sortKey="Medema, R H" uniqKey="Medema R">R. H. Medema</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tsou, M F B" uniqKey="Tsou M">M-F. B. Tsou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marschall, L G" uniqKey="Marschall L">L. G. Marschall</name></author><author><name sortKey="Jeng, R L" uniqKey="Jeng R">R. L. Jeng</name></author><author><name sortKey="Mulholland, J" uniqKey="Mulholland J">J. Mulholland</name></author><author><name sortKey="Stearns, T" uniqKey="Stearns T">T. Stearns</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kobayashi, T" uniqKey="Kobayashi T">T. Kobayashi</name></author><author><name sortKey="Tsang, W Y" uniqKey="Tsang W">W. Y. Tsang</name></author><author><name sortKey="Li, J" uniqKey="Li J">J. Li</name></author><author><name sortKey="Lane, W" uniqKey="Lane W">W. Lane</name></author><author><name sortKey="Dynlacht, B D" uniqKey="Dynlacht B">B. D. Dynlacht</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Leisner, C" uniqKey="Leisner C">C. Leisner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yamashita, Y M" uniqKey="Yamashita Y">Y. M. Yamashita</name></author><author><name sortKey="Fuller, M T" uniqKey="Fuller M">M. T. Fuller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Campbell, E K M" uniqKey="Campbell E">E. K. M. Campbell</name></author><author><name sortKey="Werts, A D" uniqKey="Werts A">A. D. Werts</name></author><author><name sortKey="Goldstein, B" uniqKey="Goldstein B">B. Goldstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Caydasi, A K" uniqKey="Caydasi A">A. K. Caydasi</name></author><author><name sortKey="Ibrahim, B" uniqKey="Ibrahim B">B. Ibrahim</name></author><author><name sortKey="Pereira, G" uniqKey="Pereira G">G. Pereira</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hotz, M" uniqKey="Hotz M">M. Hotz</name></author><author><name sortKey="Lengefeld, J" uniqKey="Lengefeld J">J. Lengefeld</name></author><author><name sortKey="Barral, Y" uniqKey="Barral Y">Y. Barral</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Johnson, A E" uniqKey="Johnson A">A. E. Johnson</name></author><author><name sortKey="Mccollum, D" uniqKey="Mccollum D">D. McCollum</name></author><author><name sortKey="Gould, K L" uniqKey="Gould K">K. L. Gould</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Furuta, K" uniqKey="Furuta K">K. Furuta</name></author><author><name sortKey="Edamatsu, M" uniqKey="Edamatsu M">M. Edamatsu</name></author><author><name sortKey="Maeda, T" uniqKey="Maeda T">T. Maeda</name></author><author><name sortKey="Toyoshima, Y Y" uniqKey="Toyoshima Y">Y. Y. Toyoshima</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Craik, D J" uniqKey="Craik D">D. J. Craik</name></author><author><name sortKey="Fairlie, D P" uniqKey="Fairlie D">D. P. Fairlie</name></author><author><name sortKey="Liras, S" uniqKey="Liras S">S. Liras</name></author><author><name sortKey="Price, D" uniqKey="Price D">D. Price</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mayer, T U" uniqKey="Mayer T">T. U. Mayer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, K S" uniqKey="Chan K">K.-S. Chan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Katsetos, C D" uniqKey="Katsetos C">C. D. Katsetos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Katsetos, C D" uniqKey="Katsetos C">C. D. Katsetos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, T" uniqKey="Liu T">T. Liu</name></author><author><name sortKey="Niu, Y" uniqKey="Niu Y">Y. Niu</name></author><author><name sortKey="Yu, Y" uniqKey="Yu Y">Y. Yu</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y. Liu</name></author><author><name sortKey="Zhang, F" uniqKey="Zhang F">F. Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Niu, Y" uniqKey="Niu Y">Y. Niu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Syed, M I" uniqKey="Syed M">M. I. Syed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Caracciolo, V" uniqKey="Caracciolo V">V. Caracciolo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cho, E H" uniqKey="Cho E">E. H. Cho</name></author><author><name sortKey="Whipple, R A" uniqKey="Whipple R">R. A. Whipple</name></author><author><name sortKey="Matrone, M A" uniqKey="Matrone M">M. A. Matrone</name></author><author><name sortKey="Balzer, E M" uniqKey="Balzer E">E. M. Balzer</name></author><author><name sortKey="Martin, S S" uniqKey="Martin S">S. S. Martin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Loh, J K" uniqKey="Loh J">J.-K. Loh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maounis, N F" uniqKey="Maounis N">N. F. Maounis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rath, O" uniqKey="Rath O">O. Rath</name></author><author><name sortKey="Kozielski, F" uniqKey="Kozielski F">F. Kozielski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moreno, S" uniqKey="Moreno S">S. Moreno</name></author><author><name sortKey="Klar, A" uniqKey="Klar A">A. Klar</name></author><author><name sortKey="Nurse, P" uniqKey="Nurse P">P. Nurse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maundrell, K" uniqKey="Maundrell K">K. Maundrell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y. Huang</name></author><author><name sortKey="Hamada, M" uniqKey="Hamada M">M. Hamada</name></author><author><name sortKey="Patel, J" uniqKey="Patel J">J. Patel</name></author><author><name sortKey="Maraia, R J" uniqKey="Maraia R">R. J. Maraia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hou, H" uniqKey="Hou H">H. Hou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Krawchuk, M D" uniqKey="Krawchuk M">M. D. Krawchuk</name></author><author><name sortKey="Wahls, W P" uniqKey="Wahls W">W. P. Wahls</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woods, A" uniqKey="Woods A">A. Woods</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Samejima, I" uniqKey="Samejima I">I. Samejima</name></author><author><name sortKey="Lourenco, P C C" uniqKey="Lourenco P">P. C. C. Lourenco</name></author><author><name sortKey="Snaith, H A" uniqKey="Snaith H">H. A. Snaith</name></author><author><name sortKey="Sawin, K E" uniqKey="Sawin K">K. E. Sawin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Burns, R G" uniqKey="Burns R">R. G. Burns</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Olmsted, Zachary T" sort="Olmsted, Zachary T" uniqKey="Olmsted Z" first="Zachary T." last="Olmsted">Zachary T. Olmsted</name></noRegion><name sortKey="Colliver, Andrew G" sort="Colliver, Andrew G" uniqKey="Colliver A" first="Andrew G." last="Colliver">Andrew G. Colliver</name><name sortKey="Paluh, Janet L" sort="Paluh, Janet L" uniqKey="Paluh J" first="Janet L." last="Paluh">Janet L. Paluh</name><name sortKey="Riehlman, Timothy D" sort="Riehlman, Timothy D" uniqKey="Riehlman T" first="Timothy D." last="Riehlman">Timothy D. Riehlman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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